Single inflorescences of litchi trees produce several hundred flowers each. The sexes are separated on different flowers and male, female and male flowers are produced successively on a given inflorescence. Self-pollination occurs commonly since pollen shed overlaps with receptivity of the female flowers. Two methods, daily physical removal of the male flowers prior to hand pollination, and the use of a pollen dispenser attached to a beehive inside an encaged tree were used to produce directed crosses as part of a litchi breeding programme. The success of the two methods was investigated with isozyme analysis of the parents and progeny. Both methods resulted in the production of a satisfactory percentage of hybrid progeny. Physical removal of the male flowers followed by hand pollination is, however, the preferred method since it resulted in a minimum of 75% hybrid progeny.